PhyloAssigner 6.166 – Phylogenetic Classification of Amplicon Pyrosequences

PhyloAssigner 6.166

:: DESCRIPTION

PhyloAssigner is a Perl pipeline for phylogenetic classification of amplicon pyrosequences.

::DEVELOPER

Bioinformatics at AWI

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux
  • Perl

:: DOWNLOAD

PhyloAssigner

:: MORE INFORMATION

Citation

ISME J. 2013 Jul;7(7):1322-32. doi: 10.1038/ismej.2013.32.
High-resolution SAR11 ecotype dynamics at the Bermuda Atlantic Time-series Study site by phylogenetic placement of pyrosequences.
Vergin KL1, Beszteri B, Monier A, Thrash JC, Temperton B, Treusch AH, Kilpert F, Worden AZ, Giovannoni SJ.

AbundantOTU+ 0.93b – Identification and Quantification of Abundant Species from Pyrosequences of 16S rRNA

AbundantOTU+ 0.93b

:: DESCRIPTION

AbundantOTU+ deals with sequences from rare species as well, compared to AbundantOTU!!

AbundantOTU is a software based on a Consensus Alignment (CA) algorithm,which infers consensus sequences, each representing an OTU,taking advantage of the sequence redundancy for abundant species. Pyrosequencing reads can then be recruited to the consensus sequences to give quantitative information for the corresponding species. As tested on 16S rRNA pyrosequence datasets from mock communities with known species, AbundantOTU rapidly reported identiffied sequences of the source 16S rRNAs and the abundances of the corresponding species.AbundantOTU was also applied to 16S rRNA pyrosequence datasets derived from real microbial communities and the results are in general agreement with previous studies.

::DEVELOPER

Yuzhen Ye lab

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux
  • Python

:: DOWNLOAD

 AbundantOTU+

:: MORE INFORMATION

Citation:

Yuzhen Ye.
Identification and quantification of abundant species from pyrosequences of 16S rRNA by consensus alignment.
The Proceedings of BIBM 2010, 153-157

ESPRIT 201102 – Estimating Species Richness Using Large Collections of 16S rRNA Pyrosequences

ESPRIT 201102

:: DESCRIPTION

ESPRIT is an algorithm for estimating species richness using large collections of 16S rRNA pyrosequences.

::DEVELOPER

Bioinformatics Laboratory, SUNY Buffalo

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux / Windows

:: DOWNLOAD

 ESPRIT

:: MORE INFORMATION

Citation:

Y. Sun, Y. Cai, L. Liu, F. Yu, M. L. Farrell, W. McKendree, and W. Farmerie,
ESPRIT: Estimating Species Richness Using Large Collections of 16S rRNA Pyrosequences,
Nucleic Acids Research, vol. 37, no. 10, e76, 2009. (impact factor: 7.8)