SGCaller – Call Genotypes in Sequencing Traces

SGCaller

:: DESCRIPTION

SGCaller is a Windows program that calls genotypes in sequencing traces.The software reads a collection of trace files in ABI or Staden (SCF) format, finds the variant position and tries to call the genotype for each, and presents them for the user to confirm and enhance the calling. SGCaller determines each file’s read direction from its name. After aligning sample traces to the reference, SGCaller calculates the total area under all four peaks for the base to be genotyped. Peak boundaries from the trace file are used; traces are not normalized to one another.

Advertisement

::DEVELOPER

Institute for Clinical Molecular Biology

:: SCREENSHOTS

::REQUIREMENTS

  • Windows
  • Microsoft Universal Data Access (MDAC)

:: DOWNLOAD

 SGCaller

:: MORE INFORMATION

Citation

Manaster C, Valentonyte R, Teuber M, Zheng W, Schreiber S, Hampe J (2005).
SGCaller: A program to call and review genotypes measured by sequencing.
BioTechniques 38(4): 544-546

Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.