NucPred (pronounced newk-pred) analyses a eukaryotic protein sequence and predicts if the protein: spends at least some time in the nucleus or spends no time in the nucleus. Don’t forget that proteins can have multiple functions and/or multiple subcellular locations. However, if a protein is already known to be secreted or is an integral membrane protein, a second role as a nuclear protein is not likely. NucPred will make a small number of confident but contradictory predictions like this. So please use all sources of biological information (both real and predicted) when interpreting the results.