Bareback 1.0
:: DESCRIPTION
Bareback is a tool to shuffle Illumina image files with low complexity sequence at the start of all sequences to the end.Bareback-processing allows the user to ‘move’ the raw images acquired by the Illumina Genome Analyser IIx from initial cycles with a very biased sequence composition to the end of the run. For samples with very biased initial sequences (such as restriction enzyme sites) and/or very high cluster densities this procedure can allow to a dramatic increase in sequence yield. This approach also works very well for custom-made multiplexing applications.
:: DEVELOPER
:: SCREENSHOTS
N/A
:: REQUIREMENTS
- Linux/ Windows /Mac OsX
- Perl
:: DOWNLOAD
:: MORE INFORMATION
Citation
PLoS One. 2011 Jan 28;6(1):e16607.
Large scale loss of data in low-diversity illumina sequencing libraries can be recovered by deferred cluster calling.
Krueger F, Andrews SR, Osborne CS.