GenomeMasker 1.3 / GenomeTester 1.3 / SNPmasker 1.1 – Design Unique Genomic PCR Primers

GenomeMasker 1.3 / GenomeTester 1.3 / SNPmasker 1.1

:: DESCRIPTION

The GENOMEMASKER package is able to mask the entire human genome for non-unique primers within 6 hours and find locations of all binding sites for 10 000 designed primer pairs within 10 minutes. Additionally, it predicts all alternative PCR products from large genomes for given primer pairs.

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GenomeTester (gtester) is the program that tests 1) whether PCR primers have excessive number of binding sites on template sequence and 2) how many PCR products would be amplified from the template DNA and where are they located.

SNPmasker is a program to mask all SNPs in given sequence using information of dbSNP

::DEVELOPER

Department of Bioinformatics, University of Tartu

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux

:: DOWNLOAD

 GENOMEMASKER

:: MORE INFORMATION

Citation:

Methods Mol Biol. 2015;1275:1-16. doi: 10.1007/978-1-4939-2365-6_1.
Fast masking of repeated primer binding sites in eukaryotic genomes.
Andreson R1, Kaplinski L, Remm M.

Andreson R., Reppo E., Kaplinski L., Remm M.
GENOMEMASKER package for designing unique genomic PCR primers.
BMC Bioinformatics 2006, 7:172.

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