Casboundary is the first method able to automatically define CRISPR cassette boundaries. In addition, it contains a Cas type predictive model used to assign each gene located in the region defined by a cassette’s boundaries a Cas label from a set of pre-defined Cas types. Furthermore, the proposed method can detect potentially new cas genes and decompose a cassette into its modules.
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Padilha VA, Alkhnbashi OS, Tran VD, Shah SA, Carvalho ACPLF, Backofen R.
Casboundary: automated definition of integral Cas cassettes.
Bioinformatics. 2021 Jun 16;37(10):1352-1359. doi: 10.1093/bioinformatics/btaa984. PMID: 33226067; PMCID: PMC8208735.