EnzymeX 3.3.3 – Determine Restriction Enzymes

EnzymeX 3.3.3

:: DESCRIPTION

EnzymeX helps molecular biologists determine which restriction enzymes they should use to cut their DNA of interest, along with complete DNA sequence analysis and editing.

::DEVELOPER

Nucleobytes

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  •  Mac OsX

:: DOWNLOAD

EnzymeX

:: MORE INFORMATION

REDseq 1.36.0 – Analysis of High-throughput Sequencing data processed by Restriction Enzyme Digestion

REDseq 1.36.0

:: DESCRIPTION

REDseq includes functions to build restriction enzyme cut site (RECS) map, distribute mapped sequences on the map with five different approaches, find enriched/depleted RECSs for a sample, and identify differentially enriched/depleted RECSs between samples.

::DEVELOPER

Program in Gene Function and Expression@umassmed

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Windows/Linux/MacOsX
  • BioConductor
  • R package

:: DOWNLOAD

 REDseq

:: MORE INFORMATION

Citation:

Chen PB, Zhu LJ, Hainer SJ, McCannell KN, Fazzio TG.
Unbiased chromatin accessibility profiling by RED-seq uncovers unique features of nucleosome variants in vivo.
BMC Genomics. 2014 Dec 15;15(1):1104. doi: 10.1186/1471-2164-15-1104. PMID: 25494698; PMCID: PMC4378318.

Mutant Design – Restriction Enzyme / Codon Optimization for Mutant Protein Design

Mutant Design

:: DESCRIPTION

Mutant Design is an excel macro of restriction enzyme / codon optimization for mutant protein design.

::DEVELOPER

Institute for Genomics and Bioinformatics, University of California

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Windows/ MacOsX
  • Excel

:: DOWNLOAD

  Mutant Design

:: MORE INFORMATION

REPK 1.3 – Restriction Enzyme Picker

REPK 1.3

:: DESCRIPTION

REPK (Restriction Endonuclease Picker) finds sets of 4 commercially available restriction endonucleases which together uniquely differentiate designated sequence groups from a supplied FASTA format sequence file for use in T-RFLP. If you have a database of known sequences from an environment, this program could be useful to pick some enzymes that uniquely discriminate the different groups in your database.

::DEVELOPER

the Rocap lab

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux/MacOsX

:: DOWNLOAD

 REPK

:: MORE INFORMATION

Citation:

Collins, R. E. and G. Rocap. 2007.
REPK: an analytical web server to select restriction endonucleases for terminal restriction fragment length polymorphism analysis.
Nucleic Acids Res. 35 (Database issue): W58-W62; doi:10.1093/nar/gkm384

 

REtools 5.0-101 – Assist in Restriction Enzyme Use

REtools 5.0-101

:: DESCRIPTION

REtools is a user friendly and easily customized program to organize any laboratory enzyme stock, brings a software solution that makes restriction enzyme use and reaction condition determination straightforward and efficient.

The usually unexplored potential of isoschizomers becomes accessible to all, since REtools proposes all possible enzymes similar to the one(s) chosen by the user. Finally, many of the commonly overlooked subtleties of restriction enzyme work, such as methylation requirement, unusual reaction conditions, as well as the number of flanking bases required for cleavage, are automatically provided by REtools.

::DEVELOPER

P. Pognonec

:: SCREENSHOTS

:: REQUIREMENTS

  • Windows / Mac OsX

:: DOWNLOAD

REtools for Win ; for Mac ; Manual

:: MORE INFORMATION

Citation:

REtools: A laboratory program for restriction enzyme work: enzyme selection and reaction condition assistance. BMC Bioinformatics 2006 Feb; 7:98

Any comment and/or proposal concerning this application is welcome! Please contact P. Pognonec for additional information.

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