Mutagenesis Primer Generator is a simple windows GUI program to generate mutagenesis primer by inputting sequence, amino acid number, and letter of amino acid you want to mutate to.
ProbeMaker is a Java software project aimed at providing a framework for design and analysis of sets of oligonucleotide probes for use in multiplex assays for nucleic acid analyses and other purposes.
DEODAS designs & analyzes consensus-degenerate oligonucleotide probes for biological research. DEODAS automatically designs and screens, probes against databases of known genes & stores the information in a searchable database.
MultiPLX is a tool for analyzing PCR primer compatibility and automatically finding optimal multiplexing (grouping) solution. It uses state-of-the-art nearest neighbour DNA binding thermodynamics to estimate possible unwanted pairings between PCR samples.
SLICSel is a program for designing specific oligonucleotide probes for microbial detection and identification. To obtain maximal specificity of designed oligonucleotides, SLICSel uses the Nearest-Neighbor thermodynamics-based approach for probe design.
HTqPCR, a package for the R statistical computing environment, is a software to enable the processing and analysis of qPCR data across multiple conditions and replicates.
StarPrimer D′Signer is a software to facilitate the design of primers for the ENTRY cloning step to create the Donor Vector. The software is also very well suited for the design of primers in combination with a site-directed mutation approach. In this case it is used to design primers for introducing mutations into your gene of interest which then is transferred into a StarGate ENTRY vector to create a Donor Vector carrying the mutated gene of interest.
QPCR is a versatile web-based Java application that allows to store, manage, and analyze data from quantitative real-time polymerase chain reaction (qPCR) experiments. It comprises a parser to automatically import generated data from qPCR devices and includes a variety of analysis methods to calculate Ct and amplification efficiency values. The analysis pipeline includes technical and biological replicate handling, incorporates sample or gene specific efficiency, normalization using single or multiple housekeeping genes, inter-run calibration, and fold change calculation. Moreover, the application supports proper error propagation throughout all analysis steps and allows conducting statistical tests on sample classes. Results can be visualized in highly customizable charts and exported for further investigation.