The rlsim package is a collection of tools for simulating RNA-seq library construction, aiming to reproduce the most important factors which are known to introduce significant biases in the currently used protocols: hexamer priming, PCR amplification and size selection.
Botond Sipos, Tim Massingham and Nick Goldman (2013):
rlsim – a package for simulating RNA-seq library preparation with parameter estimation.
http://bit.ly/rlsim-doc
The Virtual Twitch is a simulation of the rat phrenic nerve-hemidiaphragm preparation, used to the study the actions of neuromuscular blocking and reversal agents, and other drugs which affect neuromuscular transmission. The hemidiaphraghm is a large, focally innervated, respiratory skeletal muscle, composed of fast-type muscle fibres. Electrical stimulation of the phrenic nerve evokes fast, shorting-lasting muscle twitches.
BUFFER is a freeware program that accepts a valid pH value and returns one or more methods of preparation of suitable buffers and a number of pH-indicators that change colors close to the pH value entered.