TSpred – Predict Substitutions that Transform Query Protein Sequence/structure into Temperature-sensitive Mutant

TSpred

:: DESCRIPTION

TSpred attempts to identify a small set of amino acid residues in a query protein that have a high probability of being buried (side-chain accessibilities less than 5%, expressed in terms of residue depth). The server suggests substitutions at these buried positions that are most likely to result in a temperature sensitive (Ts) phenotype.

::DEVELOPER

Bioinformatics Institute of Singapore.

:: SCREENSHOTS

N/A

:: REQUIREMENTS

Web Browser

:: DOWNLOAD

:: MORE INFORMATION

Citation:

Nucleic Acids Res. 2014 Apr 29. [Epub ahead of print]
TSpred: a web server for the rational design of temperature-sensitive mutants.
Tan KP1, Khare S, Varadarajan R, Madhusudhan MS.

Tn-seq Explorer 1.5B – Analysis of High-throughput Sequencing data of Transposon Mutant Libraries

Tn-seq Explorer 1.5B

:: DESCRIPTION

Tn-seq Explorer allows users to explore and analyze Tn-seq data for prokaryotic (bacterial or archaeal) genomes.

::DEVELOPER

Computational Microbiology Laboratory

:: SCREENSHOTS

N/A

:: REQUIREMENTS

Linux/Windows
JAVA

:: DOWNLOAD

Tn-seq Explorer

:: MORE INFORMATION

Citation

Tn-seq explorer: a tool for analysis of high-throughput sequencing data of transposon mutant libraries.
Solaimanpour S, Sarmiento F, Mrázek J.
PLoS One. 2015 May 4;10(5):e0126070. doi: 10.1371/journal.pone.0126070

MAQGene 0.9.4 – Pipeline for Mutant Discovery

MAQGene 0.9.4

:: DESCRIPTION

MAQGene is a software for C. elegans biologists with next-gen sequence data, facilitates genome-wide discovery of biologically meaningful mutations.MAQGene classifies each found mutation based on its canonically predicted effect on the coding sequence.

::DEVELOPER

Hobert lab

:: SCREENSHOTS

N/A

:: REQUIREMENTS

Linux
Web Server

:: DOWNLOAD

MAQGene

:: MORE INFORMATION

Citation

Nat Methods. 2009 Aug;6(8):549. doi: 10.1038/nmeth.f.260.
MAQGene: software to facilitate C. elegans mutant genome sequence analysis.
Bigelow H, Doitsidou M, Sarin S, Hobert O.