Tag: FCS

Hackit – Program for Clipping & Joining FCS List Mode Data Files

Hackit

:: DESCRIPTION

Hackit is a program for clipping and joining FCS list mode data files.Where it is feared that a flow cytometry data file has been violated, for example at the start of the file by cells carried over from the previous sample, or at the end of the file by a nozzle blockage, Hackit can be used to clip the offending stream of cells from the file. It may be used also to concatenate the data from a number of files.

::DEVELOPER

Francis L. Battye

:: SCREENSHOTS

:: REQUIREMENTS

  • Windows / Mac OsX /  Linux
  • Java

:: DOWNLOAD

 Hackit

:: MORE INFORMATION

PyCorrFit 1.1.7 – Data Analysis software for Fluorescence Correlation Spectroscopy (FCS).

PyCorrFit 1.1.7

:: DESCRIPTION

PyCorrFit is a general-purpose FCS evaluation software that, amongst other formats, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-in model functions, covering a wide range of applications in standard confocal FCS. In addition, it contains equations dealing with different excitation geometries like total internal reflection (TIR).

::DEVELOPER

Paul Müller

:: SCREENSHOTS

:: REQUIREMENTS

  • Linux/ WIndows/ MacOsX
  • Python

:: DOWNLOAD

  PyCorrFit

:: MORE INFORMATION

Citation

Bioinformatics. 2014 May 13. pii: btu328. [Epub ahead of print]
PyCorrFit – generic data evaluation for fluorescence correlation spectroscopy.
Müller P1, Schwille P2, Weidemann T1.

PyScanFCS 0.3.6 – Analysis of perpendicular Line Scanning FCS.

PyScanFCS 0.3.6

:: DESCRIPTION

PyScanFCS interactively extracts the fluctuating fluorescence signal from such measurements and applies a multiple-tau algorithm.

::DEVELOPER

Paul Müller

:: SCREENSHOTS

 

:: REQUIREMENTS

  • Linux/ WIndows/ MacOsX
  • Python

:: DOWNLOAD

 PyScanFCS

:: MORE INFORMATION

Citation

Methods Mol Biol. 2014;1076:635-51. doi: 10.1007/978-1-62703-649-8_29.
Scanning fluorescence correlation spectroscopy (SFCS) with a scan path perpendicular to the membrane plane.
Müller P1, Schwille P, Weidemann T.

FoCuS-point 1.16.197 – Python TCSPC FCS data Visualiser

FoCuS-point 1.16.197

:: DESCRIPTION

FoCuS-point utilises advanced time-correlated single-photon counting (TCSPC) correlation algorithms along with time-gated filtering and innovative data visualisation.

::DEVELOPER

Dominic Waithe

:: SCREENSHOTS

N/A

::REQUIREMENTS

  • Windows/Linux/MacOsX
  • Python

:: DOWNLOAD

 FoCuS-point

:: MORE INFORMATION

Citation

FoCuS-point: Software for STED Fluorescence Correlation and Time-Gated Single Photon Counting.
Waithe D, Clausen MP, Sezgin E, Eggeling C.
Bioinformatics. 2015 Nov 20. pii: btv687.

Ferdinand 3.10 – Read and analyze FCS 3.0 data files collected by Flow Cytometers

Ferdinand 3.10

:: DESCRIPTION

Ferdinand is a freeware stand-alone program that reads FCS 3.0 data files and analyzes them from the distributions of fluorescent events. Ferdinand performs a number of analytical tasks when analyzing data collected by Flow Cytometers, machines which measure the optical and fluorescence properties of particles as they pass through lasers.

::DEVELOPER

Lawrence’s Lab

:: SCREENSHOTS

:: REQUIREMENTS

  • Windows

:: DOWNLOAD

 Ferdinand

:: MORE INFORMATION

TestDNA – Generates FCS Histograms of Cell Cycle Data

TestDNA

:: DESCRIPTION

TestDNA is a utility that generates FCS histograms of cell cycle data with known component values. Using its batch mode you can generate thousands of histogram files with the click of a button.

::DEVELOPER

Verity Software House

:: SCREENSHOTS

:: REQUIREMENTS

  • Windows

:: DOWNLOAD

TestDNA

:: MORE INFORMATION

FCSViewer 2.0 – Decompresses FCS data files for Display

FCSViewer 2.0

:: DESCRIPTION

FCSViewer program is to decompress a file with Zeiss Fluorescence Correlation Spectroscopy (FCS) data (in either ConfoCor 2 or ConfoCor 3 formats) and display the two channels.  In addition, time “calipers” can be used to select and enlarge any time interval, and this can be repeated until the full resolution of the data is displayed.

::DEVELOPER

Earl F Glynn

:: SCREENSHOTS

:: REQUIREMENTS

  • Windows

:: DOWNLOAD

  FCSViewer , Source Code, for R

:: MORE INFORMATION

Distributed under GNU General Public License
www.gnu.org/licenses/gpl.txt

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