The program ‘sff2fastq‘ extracts read information from a SFF file,produced by the 454 genome sequencer, and outputs the sequences and quality scores in a FASTQ format.
SFF Workbench (Standard flowgram format , SFF Tools) is an easy to use SFF file viewer, editor and converter. It is the first and only SFF editor with graphic interface on the entire bioinformatics market! With this tool we wanted to help the biologists to concentrate on their work instead of wasting time poking commands in an obscure text console.
The Roche/454 platform produces sequence reads in SFF format. SFF file contains the flow grams and quality scores for each read in addition to other information.