SAAP-BS 1.0 – Streamlined Analysis and Annotation Pipeline for RRBS

SAAP-BS 1.0

:: DESCRIPTION

SAAP-BS (or  SAAP-RRBS) Streamlined Analysis and Annotation Pipeline for Reduced Representation Bisulfite Sequencing) offers an end-to-end solution for analyzing and interpreting next-generation methylation sequencing data. The software integrates read quality assessment/clean-up, alignment, methylation data extraction, annotation, reporting, and visualization. With this package, bioinformaticians or investigators can start from sequencing reads and get a fully annotated CpG methylation report quickly allowing more time for biological interpretation.

::DEVELOPER

Bioinformatics Program, Division of Biomedical Statistics and Informatics, Mayo Clinic Research

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux
  • Perl
  • Java
  • Python

:: DOWNLOAD

  SAAP-RRBS

:: MORE INFORMATION

Citation

Bioinformatics. 2012 Aug 15;28(16):2180-1. Epub 2012 Jun 10.
SAAP-RRBS: streamlined analysis and annotation pipeline for reduced representation bisulfite sequencing.
Sun Z, Baheti S, Middha S, Kanwar R, Zhang Y, Li X, Beutler AS, Klee E, Asmann YW, Thompson EA, Kocher JP.

TRACE-RRBS v0.1 – Targeted Alignment and Artificial Cytosine Elimination for RRBS

TRACE-RRBS v0.1

:: DESCRIPTION

TRACE-RRBS is a full pipeline for mapping bisulfite se-quencing data using bowtie2 and then generating CpG level data.

::DEVELOPER

Bioinformatics Program, Division of Biomedical Statistics and Informatics, Mayo Clinic Research

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux / VirtualBox

:: DOWNLOAD

 TRACE-RRBS

:: MORE INFORMATION

Citation

Targeted alignment and end repair elimination increase alignment and methylation measure accuracy for reduced representation bisulfite sequencing data.
Baheti S, Kanwar R, Goelzenleuchter M, Kocher JP, Beutler AS, Sun Z.
BMC Genomics. 2016 Feb 27;17(1):149. doi: 10.1186/s12864-016-2494-8.