dsCheck — Off-target Search website for dsRNA-mediated RNAi

dsCheck

:: DESCRIPTION

dsCheck is a web-based online software for estimating off-target effects caused by the long double-stranded RNA (dsRNA) used in RNAi studies.

::DEVELOPER

Morishita Laboratory

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Web Browser

:: DOWNLOAD

 NO

:: MORE INFORMATION

Citation:

Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W589-91.
dsCheck: highly sensitive off-target search software for double-stranded RNA-mediated RNA interference.
Naito Y, Yamada T, Matsumiya T, Ui-Tei K, Saigo K, Morishita S.

RNAinteract 1.40.0 / RNAinteractMAPK 1.30.0 – Mapping of Signalling Networks through Synthetic Genetic Interaction Analysis by RNAi

RNAinteract 1.40.0 / RNAinteractMAPK 1.30.0

:: DESCRIPTION

RNAinteract estimates genetic interactions from multi-dimensional read-outs like features extracted from images.

RNAinteractMAPK is a software of Mapping of Signalling Networks through Synthetic Genetic Interaction Analysis by RNAi

::DEVELOPER

RNAinteract team

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux/ Windows/MacOsX
  • R package
  • Bioconductor

:: DOWNLOAD

  RNAinteract  / RNAinteractMAPK 

:: MORE INFORMATION

Citation

Thomas Horn, Thomas Sandmann, Bernd Fischer, Elin Axelsson, Wolfgang Huber, Michael Boutros,
Mapping of signaling networks through synthetic genetic interaction analysis by RNAi,
Nature methods 8 (4), 341-346, 2011.

SI-FI 1.2.3 – RNAi (RNA interference) Off-target Prediction

SI-FI 1.2.3

:: DESCRIPTION

si-Fi (siRNA Finder) is a Software for RNAi (RNA interference) off-target prediction.The software is designed for checking long-double stranded RNAi constructs for target specificity and silencing efficiency. It can be used also to select sequence regions suitable for designing RNAi constructs. The program provides clear tabular and graphical outputs.

::DEVELOPER

Stefanie Lück

:: SCREENSHOTS

:: REQUIREMENTS

  • Windows

:: DOWNLOAD

 SI-FI

:: MORE INFORMATION

Citation

Lück S, Kreszies T, Strickert M, Schweizer P, Kuhlmann M, Douchkov D.
siRNA-Finder (si-Fi) Software for RNAi-Target Design and Off-Target Prediction.
Front Plant Sci. 2019 Aug 15;10:1023. doi: 10.3389/fpls.2019.01023. PMID: 31475020; PMCID: PMC6704232.

cellHTS2 2.57.0 – Analysis of Cell-based RNAi Screens

cellHTS2 2.57.0

:: DESCRIPTION

cellHTS2 is a software package implemented in Bioconductor/R to analyze cell-based high-throughput RNAi screens.

::DEVELOPER

Boutros group & Huber Group

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux/ WIndows/MacOsX
  • R package
  • BioConductor
  • Java

:: DOWNLOAD

  cellHTS2

:: MORE INFORMATION

Citation

Genome Biol. 2006;7(7):R66.
Analysis of cell-based RNAi screens.
Boutros M, Brás LP, Huber W.

Pelz., O., Gilsdorf, M., Boutros, M. (2010).
web cellHTS2: A web application for the analysis of high-throughput screening data.
BMC Bioinformatics 11:185.

E-RNAi 3.2 – Design Optimized RNAi Constructs

E-RNAi 3.2

:: DESCRIPTION

E-RNAi is a tool for the design and evaluation of RNAi reagents for a variety of species. It can be used to design and evaluate long dsRNAs (including esiRNAs) as well as siRNAs.

::DEVELOPER

E-RNAi team

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Web Browser 

:: DOWNLOAD

 NO

:: MORE INFORMATION

Citation

E-RNAi: a web application for the multi-species design of RNAi reagents–2010 update.
Thomas Horn; Michael Boutros
Nucl. Acids Res. (2010) 38 (suppl 2): W332-W339. doi: 10.1093/nar/gkq317

SbacHTS v5 – Spatial background correction for High-Throughput RNAi Screening

SbacHTS v5

:: DESCRIPTION

SbacHTS is a software for visualization, estimation and correction of spatial background noises of RNAi screening experiment results.

::DEVELOPER

The Quantitative Biomedical Research Center 

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux / Windows/ MacOsX
  • R

:: DOWNLOAD

 SbacHTS

:: MORE INFORMATION

Citation

Bioinformatics. 2013 Sep 1;29(17):2218-20. doi: 10.1093/bioinformatics/btt358. Epub 2013 Jun 28.
SbacHTS: spatial background noise correction for high-throughput RNAi screening.
Zhong R1, Kim MS, White MA, Xie Y, Xiao G.

NePhe 1.0 – Network RNAi Phenotype Score

NePhe 1.0

:: DESCRIPTION

NePhe (Network RNAi Phenotype) is intended to implement a network-based integrative approach to prioritize reliable hits in genome-wide screens

::DEVELOPER

Fengzhu Sun

:: SCREENSHOTS

N/A

:: REQUIREMENTS

:: DOWNLOAD

 NePhe

:: MORE INFORMATION

Citation

Wang L, Tu Z, Sun FZ (2009)
A network-based integrative approach to prioritize reliable hits from multiple genome-wide RNAi screens in Drosophila.
BMC Genomics 10:220.