Beacon Designer 8.21 – Automate Real Time PCR Primer & Probe Design

Beacon Designer 8.21

:: DESCRIPTION

Beacon Designer™ automates the design of real time primers and probes. It is used by molecular biologists worldwide to design successful real time PCR assays. It saves the time and the money involved in failed experiments. Beacon Designer™ is a flexible solution to your real time primer and probe design needs and pays for itself many times over.

::DEVELOPER

PREMIER Biosoft

:: SCREENSHOTS

:: REQUIREMENTS

  • MacOsX / Windows

:: DOWNLOAD

Beacon Designer Demo

:: MORE INFORMATION

Realyser 1.0 – Real Time PCR Normalisation tool

Realyser 1.0

:: DESCRIPTION

Realyser is a program to help with the selection of stable controls for QPCR and the normalisation of QPCR data. It is an application of the GNorm technique and is designed to work with the data files from ABI Real-time PCR machines.

:: DEVELOPER

Babraham Bioinformatics

:: SCREENSHOTS

:: REQUIREMENTS

  • Linux / Mac OsX/Windows
  • Java 

:: DOWNLOAD

 Realyser

:: MORE INFORMATION

SARS – Statistical Analysis of Real-time PCR Software

SARS

:: DESCRIPTION

SARS is a software package for statistical analysis of Real-time PCR data.

::DEVELOPER

Joshua Yuan lab

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux/ Windows/ MacOsX
  • Python
  • R package

:: DOWNLOAD

 SARS

:: MORE INFORMATION

Citation

Statistical analysis of real-time PCR data.
Yuan JS, Reed A, Chen F, Stewart CN Jr.
BMC Bioinformatics. 2006 Feb 22;7:85.

qbasePLUS 2.0 – Real-time PCR Data Analysis

qbasesPLUS 2.0

:: DESCRIPTION
qbasePLUS speeds up the analysis of qPCR data and improves the accuracy of your experiments. The software is the most powerful, flexible, and user-friendly real-time PCR data-analysis software based on the proven geNorm and qBase technology, enhanced with proprietary algorithms and time-saving features.

::DEVELOPER

Biogazelle

:: SCREENSHOTS

:: REQUIREMENTS

  • Linux / Windows / Mac OsX

:: DOWNLOAD

qbasePLUS

:: MORE INFORMATION

Citation

Vandesompele et al.,
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
Genome Biology, 2002

qPCR-DAMS 1.2 – Analyze, Manage, and Store Quantitative Real-Time PCR data.

qPCR-DAMS 1.2

:: DESCRIPTION

qPCR-DAMS is a database management system implemented on MS Access 2003 and Visual Basic. It is designed to analyze, manage, and store relative and absolute quantitative real-time PCR data. The system consists of 5 blocks: three blocks (Gene, Plate, and Experiment) for inputting, storing and describing raw data, and two blocks (View data and Process Data) for checking, evaluating, and processing data. Users are allowed to choose among four basic outputs: (I) Ratio relative quantification, (II) Absolute level, (III) Normalized absolute expression, and (IV) Ratio absolute quantification, and two advanced options: (V) Multiple reference relative quantification and (VI) Multiple references absolute quantification, within single software package. The coefficient of variation is monitored at each step during data processing and the accuracy is further improved by an easy data tracking and display system. In summary, qPCR-DAMS is a handy novel tool for real-time PCR users.

::DEVELOPER

Nili Jin , Keyu He and Lin Liu

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Windows
  • Microsoft Access 2000 or above

:: DOWNLOAD

 qPCR-DAMS

:: MORE INFORMATION

Citation

Nili Jin , Keyu He and Lin Liu
qPCR-DAMS: a database tool to analyze, manage, and store both relative and absolute quantitative real-time PCR data
Physiological Genomics 25:525-527 (2006)

DART-PCR 1.0 – Quantitative Real-time PCR Data Analysis

DART-PCR 1.0

:: DESCRIPTION

DART-PCR (Data Analysis for Real‐Time PCR) provides a simple means of analysing real-time PCR data from raw flurescence data. This allows an automatic calculation of amplification kinetics, as well as performing the subsequent calculations for relative quantification and calculation of assay variability. Amplification efficiencies are also tested to dtect anomalus samples within groups (outlayers) and differences between experimatal groups (amplification equivalence).

::DEVELOPER

Stuart N. Peirson, Jason N. Butler and Russell G. Foster

:: SCREENSHOTS

:: REQUIREMENTS

  • Excel

:: DOWNLOAD

 DART-PCR

:: MORE INFORMATION

Citation

Stuart N. Peirson, Jason N. Butler and Russell G. Foster (2003)
Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis
Nucl. Acids Res. (2003) 31 (14): e73