GEDpm-cg – Genome Editing Automated Design Platform for Point Mutation Construction in Corynebacterium glutamicum

GEDpm-cg

:: DESCRIPTION

GEDpm-cg enables users to perform the efficient, automated and high-throughput design of single nucleotide editing in C. glutamicum chromosome. The counter-selection HR system and the overlap-based assembly method were chosen as the loading techniques. Homologous arms and primers required for genetic modification, vector DNA assembly and sequencing verification were provided as design results.

::DEVELOPER

Biodesign Centre, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences

:: SCREENSHOTS

N/A

:: REQUIREMENTS

Web server
Python

:: DOWNLOAD

GEDpm-cg

:: MORE INFORMATION

Citation

Yang Y, Mao Y, Liu Y, Wang R, Lu H, Li H, Luo J, Wang M, Liao X, Ma H.
GEDpm-cg: Genome Editing Automated Design Platform for Point Mutation Construction in Corynebacterium glutamicum.
Front Bioeng Biotechnol. 2021 Oct 15;9:768289. doi: 10.3389/fbioe.2021.768289. PMID: 34722482; PMCID: PMC8554027.

HAPredictor – Gene-specific method for Predicting Hemophilia-causing Point Mutation

HAPredictor

:: DESCRIPTION

HApredictor is an HA-specific prediction tool which showed disease prediction accuracy comparable to other publicly available prediction software.

::DEVELOPER

Teresa Przytycka Research Group

:: SCREENSHOTS

N/A

:: REQUIREMENTS

Linux / Windows/ MacOsX
C Compiler

:: DOWNLOAD

HAPredictor

:: MORE INFORMATION

Citation

J Mol Biol. 2013 Nov 1;425(21):4023-33. doi: 10.1016/j.jmb.2013.07.037. Epub 2013 Aug 3.
A gene-specific method for predicting hemophilia-causing point mutations.
Hamasaki-Katagiri N1, Salari R, Wu A, Qi Y, Schiller T, Filiberto AC, Schisterman EF, Komar AA, Przytycka TM, Kimchi-Sarfaty C.

MAESTRO 1.2.35 / MAESTROweb – Multi Agent Stability Prediction upon point Mutations

MAESTRO 1.2.35 / MAESTROweb

:: DESCRIPTION

MAESTRO is a Multi AgEnt STability pRedictiOn tool for changes in unfolding free energy upon point mutation.

MAESTROweb provides an easy to use web interface to the MAESTRO software

::DEVELOPER

Immunoinformatics Group @ University of Salzburg

:: SCREENSHOTS

N/A

:: REQUIREMENTS

Windows / Linux

:: DOWNLOAD

MAESTRO

:: MORE INFORMATION

Citation

MAESTROweb: a web server for structure based protein stability prediction.
Laimer J, Hiebl-Flach J, Lengauer D, Lackner P.
Bioinformatics. 2016 Jan 6. pii: btv769.

MAESTRO – multi agent stability prediction upon point mutations
Laimer J, Hofer H, Fritz M, Wegenkittl S, Lackner P.
BMC Bioinformatics. 2015 Apr 16;16(1):116.

MuTect Beta – Identify Point Mutations

MuTect Beta

:: DESCRIPTION

MuTect is a method developed at the Broad Institute for the reliable and accurate identification of somatic point mutations in next generation sequencing data of cancer genomes.

::DEVELOPER

The Cancer Genome Analysis (CGA) group

:: SCREENSHOTS

N/A

:: REQUIREMENTS

Linux / Mac OsX / Windows
Java

:: DOWNLOAD

MuTect

:: MORE INFORMATION