NN50-calculator 0.5 – Evaluate the Correctness of Genome Assemblies

NN50-calculator 0.5

:: DESCRIPTION

NN50-calculator (Normalized N50 calculator) is a tool for evaluating the correctness of genome assemblies.

::DEVELOPER

Veli Mäkinen, Leena Salmela

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux
  • C++ Compiler
  • Python

:: DOWNLOAD

 NN50-calculator

:: MORE INFORMATION

Citation
BMC Bioinformatics. 2012 Oct 3;13:255. doi: 10.1186/1471-2105-13-255.
Normalized N50 assembly metric using gap-restricted co-linear chaining.
Mäkinen V, Salmela L, Ylinen J.

FastMLST v0.0.15 – Multilocus Sequence Typing of draft Genome Assemblies

FastMLST v0.0.15

:: DESCRIPTION

FastMLST is a high speed standalone script wrote in Python3, which takes assemblies in FASTA format (gzipped is also allowed) and determines its ST according to MLST schemes defined in PubMLST.

::DEVELOPER

Enzo Guerrero-Araya

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux
  • Python

:: DOWNLOAD

FastMLST

:: MORE INFORMATION

Citation

Guerrero-Araya E, Muñoz M, Rodríguez C, Paredes-Sabja D.
FastMLST: A Multi-core Tool for Multilocus Sequence Typing of Draft Genome Assemblies.
Bioinform Biol Insights. 2021 Nov 27;15:11779322211059238. doi: 10.1177/11779322211059238. PMID: 34866905; PMCID: PMC8637782.

QUAST 5.1.0 – Quality Assessment Tool for Genome Assemblies

QUAST 5.1.0

:: DESCRIPTION

QUAST evaluates a quality of genome assemblies by computing various metrics and providing nice reports.

MetaQUAST evaluates and compares metagenome assemblies based on alignments to close references.

Icarus is a novel genome visualizer for accurate assessment and analysis of genomic draft assemblies, which is based on QUAST genome quality assessment tool

::DEVELOPER

Algorithmic Biology Lab

:: SCREENSHOTS

MetaQUAST

:: REQUIREMENTS

  • Linux /  MacOsX
  • Python
  • Perl
  • MUMmer

:: DOWNLOAD

 QUAST

:: MORE INFORMATION

Citation

Icarus: visualizer for de novo assembly evaluation.
Mikheenko A, Valin G, Prjibelski A, Saveliev V, Gurevich A.
Bioinformatics. 2016 Jul 4. pii: btw379.

MetaQUAST: evaluation of metagenome assemblies.
Mikheenko A, Saveliev V, Gurevich A.
Bioinformatics. 2015 Nov 26. pii: btv697

Alexey Gurevich, Vladislav Saveliev, Nikolay Vyahhi and Glenn Tesler
QUAST: quality assessment tool for genome assemblies
Bioinformatics volume 29, issue 8, pp. 1072-1075.

GMASS – Genome Assembly Structural Similarity

GMASS

:: DESCRIPTION

The GMASS score is a novel measure for representing structural similarity between two assemblies. It represents the structural similarity of a pair of assemblies based on the length and number of similar genomic regions defined as consensus segment blocks (CSBs) in the assemblies.

::DEVELOPER

Bioinformatics Laboratory, Konkuk University

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux
  • Perl

:: DOWNLOAD

GMASS

:: MORE INFORMATION

Citation

BMC Bioinformatics. 2019 Mar 18;20(1):147. doi: 10.1186/s12859-019-2710-z.
GMASS: a novel measure for genome assembly structural similarity.
Kwon D, Lee J, Kim J.

AlignGraph – Secondary de novo Genome Assembly guided by closely related References

AlignGraph

:: DESCRIPTION

AlignGraph is a software that extends and joins contigs or scaffolds by reassembling them with help provided by a reference genome of a closely related organism.

::DEVELOPER

Girke Lab

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux

:: DOWNLOAD

 AlignGraph

:: MORE INFORMATION

Citation

Bioinformatics. 2014 Jun 15;30(12):i319-i328. doi: 10.1093/bioinformatics/btu291.
AlignGraph: algorithm for secondary de novo genome assembly guided by closely related references.
Bao E, Jiang T, Girke T.

Chromonomer 1.13 – Genome Assembly with Genetic Map

Chromonomer 1.13

:: DESCRIPTION

Chromonomer is a program designed to integrate a genome assembly with a genetic map. Chromonomer tries very hard to identify and remove markers that are out of order in the genetic map, when considered against their local assembly order; and to identify scaffolds that have been incorrectly assembled according to the genetic map, and split those scaffolds.

DEVELOPER

Cresko labs

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux

:: DOWNLOAD

Chromonomer

:: MORE INFORMATION

Citation:

Catchen J, Amores A, Bassham S.
Chromonomer: A Tool Set for Repairing and Enhancing Assembled Genomes Through Integration of Genetic Maps and Conserved Synteny.
G3 (Bethesda). 2020 Nov 5;10(11):4115-4128. doi: 10.1534/g3.120.401485. PMID: 32912931; PMCID: PMC7642942.

BUSCO v5.2.2 – Assessing Genome Assembly and Annotation Completeness with Single-copy Orthologs

BUSCO v5.2.2

:: DESCRIPTION

BUSCO completeness assessment employs sets of Benchmarking Universal Single-Copy Orthologs from OrthoDB to provide quantitative measures of the completeness of genome assemblies, annotated gene sets, and transcriptomes in terms of expected gene content.

::DEVELOPER

Prof Zdobnov Laboratory

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux
  • Python
  • NCBI BLAST+
  • HMMER (HMMER 3.1b2)
  • Augustus 3.0.x

:: DOWNLOAD

 BUSCO

:: MORE INFORMATION

Citation

BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs.
Simão FA, Waterhouse RM, Ioannidis P, Kriventseva EV, Zdobnov EM.
Bioinformatics. 2015 Jun 9. pii: btv351.

Circlator 1.5.6 – A tool to Circularize Genome Assemblies

Circlator 1.5.6

:: DESCRIPTION

Circlator is the first tool to automate assembly circularization and produce accurate linear representations of circular sequences.

::DEVELOPER

Pathogen Informatics, Wellcome Trust Sanger Institute

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux /  MacOsX
  • Python
  • BWA version >= 0.7.12
  • prodigal version >= 2.6
  • SAMtools (version 0.1.9 or 1.2)
  • MUMmer version >= 3.23
  • SPAdes version 3.5.0, 3.6.0 or 3.6.2

:: DOWNLOAD

 Circlator

:: MORE INFORMATION

Citation

Circlator: automated circularization of genome assemblies using long sequencing reads.
Hunt M, Silva ND, Otto TD, Parkhill J, Keane JA, Harris SR.
Genome Biol. 2015 Dec 29;16(1):294. doi: 10.1186/s13059-015-0849-0.

rectangles 2.0 – Rectangle Graph for Repeat Resolution in Genome Assembly

rectangles 2.0

:: DESCRIPTION

rectangles is an ultimate tool for resolving repeats in genome assemblies.

::DEVELOPER

Algorithmic Biology Lab

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux /  MacOsX
  • Python

:: DOWNLOAD

 rectangles

:: MORE INFORMATION

Citation

Nikolay Vyahhi, Son K. Pham, Pavel Pevzner.
From de Bruijn Graphs to Rectangle Graphs for Genome Assembly,
Lecture Notes in Bioinformatics 7534 (2012), pp. 249-261.

GATB 1.4.2 – Genome Assembly & Analysis Tool Box

GATB 1.4.2

:: DESCRIPTION

GATB provides an easy way to develop efficient and fast NGS tools. GATB is a C++ library of high level functions designed to benefit from recent advances in NGS data structures for handling huge datasets of sequencing data.

::DEVELOPER

GATB team

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux / MacOsX

:: DOWNLOAD

 GATB

:: MORE INFORMATION

Citation:

GATB: Genome Assembly & Analysis Tool Box.
Drezen E, Rizk G, Chikhi R, Deltel C, Lemaitre C, Peterlongo P, Lavenier D.
Bioinformatics. 2014 Jul 1. pii: btu406