Tag: Electron Tomography

TOMOJ 2.6 – Tools for Electron Tomography

TOMOJ 2.6

:: DESCRIPTION

TomoJ is a plug-in for the now standard image analysis and processing software for optical microscopy, ImageJ.TomoJ provides a user-friendly interface for alignment, reconstruction, and combination of multiple tomographic volumes and includes the most recent algorithms for volume reconstructions used in three-dimensional electron microscopy (the algebraic reconstruction technique and simultaneous iterative reconstruction technique) as well as the commonly used approach of weighted back-projection.

::DEVELOPER

TOMOJ team

:: SCREENSHOTS

:: REQUIREMENTS

:: DOWNLOAD

 TomoJ

:: MORE INFORMATION

Citation:

BMC Bioinformatics. 2007 Aug 6;8:288.
TomoJ: tomography software for three-dimensional reconstruction in transmission electron microscopy.
Messaoudi C, Boudier T, Sanchez Sorzano CO, Marco S.

SerialEM 3.7.11 – Electron Tomography Data & Image Acquisition

SerialEM 3.7.11

:: DESCRIPTION

SerialEM is a program to acquire tilt series for electron tomography on Tecnai and JEOL microscopes. It uses an approach based on prediction of specimen position during the tilt series from the position at previous tilts. It does not count on the microscope or the specimen being particularly well-behaved, so unless a prediction appears reliable, it falls back to measuring and adjusting defocus and/or specimen position when necessary. With this method, it achieves both the robustness of the older approach to tilt series acquisition (track and focus at every tilt) and the speed of the newer precalibration approach. A 2Kx2K, 1 degree tilt series can be acquired in about 45 minutes with a single-port readout camera or 20-25 minutes with a four-port readout camera.

::DEVELOPER

The Boulder Lab for 3D Electron Microscopy

:: SCREENSHOTS

:: REQUIREMENTS

  • Windows

:: DOWNLOAD

SerialEM

:: MORE INFORMATION

Citation:

Mastronarde, D.N. 2005.
Automated electron microscope tomography using robust prediction of specimen movements.
J. Struct. Biol.152:36-51.