Tag: Cloning

Deep Threshold Tool / Rosetta Tool – Analysis of Ultra-deep Pyrosequencing and Cloning

Deep Threshold Tool / Rosetta Tool

:: DESCRIPTION

Deep Threshold Tool / Rosetta Tool is web server for analysis of ultra-deep pyrosequencing and cloning based sequencing of the basic core promoter/precore/core region of hepatitis B virus using newly developed bioinformatics tools.

::DEVELOPER

Hepatitis Virus Diversity Research Unit

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Web Browser

:: DOWNLOAD

 NO

:: MORE INFORMATION

Citation:

Yousif*, M., Bell*, T. G., Mudawi, H., Glebe, D. and Kramvis, A. (2014)
Analysis of ultra-deep pyrosequencing and cloning based sequencing of the basic core promoter/precore/core region of hepatitis B virus using newly developed bioinformatics tools.
PLoS ONE 9(4): e95377.

CloneAssistant 1.0 – automated Cloning Primer Design

CloneAssistant 1.0

:: DESCRIPTION

CloneAssistant is a standalone software, which can automatically design the cloning primers for users with full consideration of the sequence information of vectors and genes, cloning strategies, the principles of primer design, reading frame, position effects, and enzymatic reaction conditions. It can be used for restriction map analysis, ORF (open reading frame) finding, sequence alignment and complementary analysis, translation, restriction enzyme and universal buffer query, and isocaudamer analysis.

::DEVELOPER

BIS @ Zhejiang University

:: SCREENSHOTS

CloneAssistant

:: REQUIREMENTS

  • Windows

:: DOWNLOAD

 CloneAssistant

:: MORE INFORMATION

Citation

J Biotechnol. 2010 Nov;150(3):294-8. doi: 10.1016/j.jbiotec.2010.09.952.
CloneAssistant 1.0: a stand-alone software for automated cloning primer design.
Shao C, Meng Y, Lv S, Zhong W, Wang Z, Chen M.

StarPrimer D’Signer 3.0.0.3 – Design of Primers for ENTRY Cloning to Create Donor Vector

StarPrimer D’Signer 3.0.0.3

:: DESCRIPTION

StarPrimer D′Signer is a software to facilitate the design of primers for the ENTRY cloning step to create the Donor Vector. The software is also very well suited for the design of primers in combination with a site-directed mutation approach. In this case it is used to design primers for introducing mutations into your gene of interest which then is transferred into a StarGate ENTRY vector to create a Donor Vector carrying the mutated gene of interest.

::DEVELOPER

IBA GmbH

:: SCREENSHOTS

:: REQUIREMENTS

  • Windows / MacOsX
  • Microsoft Silverlight

:: DOWNLOAD

StarPrimer D’Signer

:: MORE INFORMATION

Clone Manager 0.1 – Cloning and Protein Expression LIMS

Clone Manager 0.1

:: DESCRIPTION

Clone Manager – the PSF cloning and protein expression system (LIMS) is based on an Oracle database (v. 8.1.6) and a frontend developed with Microsoft Access 97. In addition, some perl scripts and Java programs are used for batch data entry.

::DEVELOPER

Protein Structure Factory

:: REQUIREMENTS

:: DOWNLOAD

Clone Manager

:: MORE INFORMATION

Please contact Konrad Büssow for questions/comments regarding this software.