SpliceR v1.2.0 – Prediction of BE-splice sgRNAs for Base Editor mediated Knockout

SpliceR v1.2.0

:: DESCRIPTION

SpliceR predicts base editing sgRNAs to use in the disruption of splice sites for gene modulation, or knockout. This approach is especially amenable to multiplex gene editing relative to Cas9 nuclease based approaches.

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::DEVELOPER

Moriarity Lab

:: SCREENSHOTS

N/A

:: REQUIREMENTS

  • Linux / Windows/ MacOsX
  • R

:: DOWNLOAD

SpliceR

:: MORE INFORMATION

Citation

Kluesner MG, Lahr WS, Lonetree CL, Smeester BA, Qiu X, Slipek NJ, Claudio Vázquez PN, Pitzen SP, Pomeroy EJ, Vignes MJ, Lee SC, Bingea SP, Andrew AA, Webber BR, Moriarity BS.
CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells.
Nat Commun. 2021 Apr 23;12(1):2437. doi: 10.1038/s41467-021-22009-2. PMID: 33893286; PMCID: PMC8065034.

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